yrvD [2019-02-19 19:04:35]

unknown

locus: BSU27630

pI: 8.72

mw: 12.10 kDa

protein length: 107 aa

gene length: 324 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 2,825,846 2,826,169

The protein

[SW|Localization]

• cell membrane (according to Uniprot)

Expression and Regulation

Operons

• genes: [gene|4AF865F1E16187F89C1FE19BAE5C2CF2C0A53E4A|yrvC]-[gene|418A95E9DD8DE2690C1E65E2A4198E5C5AC6E6D3|yrvD]
  description: [Pubmed|22383849]

• genes: [gene|418A95E9DD8DE2690C1E65E2A4198E5C5AC6E6D3|yrvD]
  description: [Pubmed|15241682]

Biological materials

Mutant

• MGNA-B517 (yrvD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1516 NBRP B. subtilis, Japan]
• GP2099 (''[gene|4AF865F1E16187F89C1FE19BAE5C2CF2C0A53E4A|yrvC]-[gene|418A95E9DD8DE2690C1E65E2A4198E5C5AC6E6D3|yrvD]''::''zeo''), available in [SW|Jrg Stlke]'s lab
• BKE27630 ([gene|418A95E9DD8DE2690C1E65E2A4198E5C5AC6E6D3|yrvD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCTTTTCCCCCAAAC, downstream forward: _UP4_TAGTTTTCGAATATTATTTG
• BKK27630 ([gene|418A95E9DD8DE2690C1E65E2A4198E5C5AC6E6D3|yrvD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCTTTTCCCCCAAAC, downstream forward: _UP4_TAGTTTTCGAATATTATTTG
• GP2713 (''[gene|4AF865F1E16187F89C1FE19BAE5C2CF2C0A53E4A|yrvC]-[gene|418A95E9DD8DE2690C1E65E2A4198E5C5AC6E6D3|yrvD]''::''cat''), available in [SW|Jrg Stlke]'s lab

FLAG-tag construct

• GP2440 ''yrvD-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jrg Stlke]'s lab

References

• 15241682